• USE OF ANTICOAGULANTS; Many haematological tests require unclotted blood specimens.
Such a blood sample can be obtained by collecting it into a tube containing an
anticoagulant. There are various types of anticoagulants used for this purpose,
each having its advantages and disadvantages. A suitable anticoagulant should
be selected according to the investigations required.
summarises the use of
three anticoagulants most commonly used in Haematology.
Note:-It is necessary to mix the blood and the anticoagulant
by gently inverting the container several times as soon as the blood is added
into it.
Defibrinated
Blood
If blood is continuously agitated in the presence of objects
such as glass beads or paper clips during coagulation. Fibrin, which is the end
product of coagulation process, collects around these objects and is separated
from the blood sample together with platelets. Most of the red cells and white
cells. remain suspended in the serum. This prevents the removal of calcium.
This procedure is adopted when the use of anticoagulant is contraindicated e.g.
for complement activity.
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