LIST OF HEMATOLOGY TEST AND THEIR PROCEDURE

Hematology -Hematology and blood banking are very important branches of laboratory medicine. The student will be able to know the basic components of blood and their significance in normal health, by examination of blood, various types of diseases can be diagnosed.

1.Introduction

2.Haemopoiesis (Production of Blood Cells)

 3.The Complete Blood Count (CBC)

4.Examination of Peripheral Blood Smear

 5.Anaemia

 6.Miscellaneous Investigations in Haematology

7.Haemostasis and Fibrinolysis

 8.Blood Group Serology (Immunohaematology)

 9.Blood Banking, Blood Transfusion and Compatibility Testing

10. The Major Histocompatibility Complex (MHC) and the HLA System

 11.Automation in Haematology

 

1.Haematology:-

• Introduction
• Collection of Blood 
• Use ofAnticoagulants

2. Haemopoiesis (Production of Blood Cells) Main Cell Lines :-

• Erythropoiesis (Formation of Red Blood Cells)
• Leucopoiesis (Formation of White Blood Cells)
• Functions ofWhite Blood Cells
• Functions ofPlatelets

3. The Complete Blood Count (CBC) :-

• Haemoglobin (Hb)
• Estimation ofHaemoglobin
• Haemocytometry(Counting of Cells in Blood) 
• Manual Cell Counts
• Use of CountingChamber (Haemocytometer) for Cell Counting
• Red Cell Count(Manual Method)
• HematocritorPacked Cell Volume (PCV)
• Red Blood CellIndices
• ErythrocyteSedimentation Rate (ESR)
• White Cell Count(Total, TLC) (Manual Method)
• Total EosinophilCount (TEC)
• Platelet Count(Manual Method)

 4.Examination of Peripheral BloodSmear:-

• Preparation ofBlood Smear

LIST OF HEMATOLOGY TEST AND THEIR PROCEDURE

• Staining the Blood Smear
• Microscopic Examination of the Blood Film
• The DifferentialCount of Leucocytes
• Interpretation of the Variations
• Abnormalities inLeucocytes
• MorphologicalVariations/Abnormalities in Erythrocytes

 5. Anemia:-

• Classification of Anemia
• Morphological Classification of Anemia
• Etiological Classification of Anemia
• PhysiologicalTypes of Anaemia

6. Miscellaneous Investigations in Hematology Reticulocyte Count :-

• Staining of Heinz Bodies
• Staining ofSiderocytes (Demonstration of Pappenheimer Bodies)
• PeroxidaseStaining  
• Periodic-AcidSchiff (PAS)Reaction
• Neutrophil (Leucocyte) Alkaline Phosphatase
• Examination ofBone Marrow
• Test for Sickle cells
• Demonstration ofLe Cells
• Osmotic Fragility Test
• Estimation ofHaemoglobin F (Foetal Haemoglobin) by Alkali Denaturation Method
• HaemoglobinElectrophoresis
• Detection ofGlucose-6-Phosphate Dehydrogenase (G-6-PD) Deficiency
• Ham's Test(Acidified Serum Test)
• Donath-Landsteiner(D-L) Antibody Test  
• Detection ofDrug-Induced Antibody
• DirectAntiglobulin Test (Direct Coomb's Test)
• Autohaemolysis test
• Spectroscopic Examination of Blood for theDetection of Abnormal Haemoglobin Pigments

7.Haemostasis and Fibrinolysis :-

• Haemostasis
• Mechanism ofHaemostasis
• Function of Plateletsin Haemostasis and Coagulation
• Coagulation
• CoagulationFactors
• Pathways for theCoagulation Process
• Fibrinolysis
• Disorders ofBlood Coagulation
• Tests forCoagulation Function
• Clotting Time(Lee-White method)
• Clot Retraction
• Test to Measurethe Extrinsic System: One-Stage Prothrombin Time (PT)  
• Test for ProthrombinConsumption Index (PCI)
• Test forIntrinsic System
• Assays of Coagulation Factors

8. Blood Group Serology:-

• (Immunohaematology) Blood Group Genetics  
• Blood Group Antigens
• Blood Group Antibodies
• Main Blood Group Systems
• ABO Blood Group System
• ABO Grouping
• ABO Grouping Methods
• Rhesus (Rh) Grouping System  
• Method 1: Saline Method for  Rh-D Typing Using Complete Anti-D
• Method 2: Albumin Displacement Technique for Rh Typing Using Incomplete Anti-D  
• Method 3: EnzymeTechniques
• AntiglobulinTechniques
• Antihuman GlobulinTests (Coombs' Tests)
• Clinically Less Significant Blood Group Systems

 10. Automation in Haematology  :-

• Automated Cell Counting Methods
• Autoanalysers in Coagulation Studies  
• Automation in Blood Group Serology