Test for B-hydroxybutyric acid

Test for B-hydroxybutyric acid

There is no direct routine test for this ketone body. Its presence in urine can be detected indirectly by first getting rid of the acetone and acetoacetic acid. Determine the urine pH; if acid, then boil to remove the acetone and acetoacetic acid.

Treat the urine with hydrogen peroxide to oxidise B-hydroxybutyric acid to acetoacetic acid.Perform the nitroprusside test. A positive reaction is indicative of the presence of B-hydroxybutyric acid.

Causes of ketonuria

1. Diabetes indicates ketoacidosis which maylead to coma if unchecked.

2. Acute febrile states especially in infants.

3. Excessive vomiting and diarrhoea. .

4. Glycogen storage impairment.